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Sterilization is an essential technique deployed across a variety of sectors, from the healthcare and pharmaceutical industries to food production. The practice eliminates, neutralizes or renders inactive all micro-organisms including fungi, bacteria, spores and single-celled eukaryotic organisms. Ensuring efficacy of sterilization is of utmost importance in guaranteeing the quality and safe use of products while minimizing the risk of disease transmission. Indicators of sterility are specifically devised to reliably assess, oversee and monitor the efficacy of cleaning, disinfection and sterilization processes.